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  • Mutation Breeding for Resistance to Striga Parasitic Weeds in Cereals for Food Security

    Closed for Proposals

    Project Type

    Coordinated Research Project

    Project Code

    D25005

    CRP

    2166

    Approved Date

    9 December 2015

    Project Status

    Closed

    Start Date

    6 May 2016

    Expected End Date

    5 May 2022

    Completed Date

    23 June 2021

    Participating Countries

    Burkina Faso, China, Ethiopia, Iran, Islamic Republic of, Italy, Japan, Kenya, Madagascar, Kingdom of the Netherlands, Sudan, Türkiye, United States of America

    Description

    The parasitic weeds Striga asiatica and S. hermonthica are major biological constraints to cereal production in most of sub-Saharan Africa and semi-arid tropical regions of Asia. Yield losses are mainly in maize, millet, rice and sorghum and most severe on marginal lands of subsistence farmers. The unique biology of Striga, tightly adapted to its crop hosts, limits control options. Because subsistence farmers either cannot afford or do not have access to inputs, host plant resistance is a vital part of Striga control. Resistance traits, however, are lacking in most cereal varieties sown in Striga prone areas. This CRP proposes the use of physical mutagenesis and associated screening technologies to broaden the genetic base of resistance. The project seeks to link cereal improvement programs in the Member States with Striga biologists and IAEA staff. This would combine expertise in screen house and laboratory screening to enhance mutation breeding to timely develop or adapt screening packages to generate novel sources of Striga resistance. Initial focus will be on sorghum and rice because of their importance to food security and amenability to mutation breeding techniques. Up to ten research contracts are expected to be awarded and five no-cost agreement holders from advanced laboratories and research institutes with recognized expertise in the targeted technologies will be invited to share their experience with the contract holders and contribute to the development and validation of the planned technical packages. In addition, it is foreseen that two technical contracts will be awarded for services in advanced areas such as marker development and advanced efficiency enhancing technologies and mutant characterization. Coordination and technical management will be handled by the scientific secretary in the Plant Breeding and Genetics Laboratory/Section.

    Objectives

    To support generation of novel sources of variation, using mutation breeding, by developing efficient screening protocols for Striga resistance in cereals for building research capacity and improving food security in Member States.

    Specific Objectives

    To develop, optimize, validate and disseminate robust screening protocols for mutant populations for resistance/tolerance to the parasitic weed Striga in major cereal crops.

    To integrate and adapt efficiency enhancing techniques in the mutation breeding processes.

    To generate genetic diversity to develop resistant/tolerant varieties to Striga infestation.

    To improve capacity in efficient mutation breeding for resistance to parasitic weeds in cereals.

    To develop, optimize, validate and disseminate robust screening protocols for mutant populations for resistance/tolerance to the parasitic weed Striga in major cereal crops.

    To integrate and adapt efficiency enhancing techniques in the mutation breeding processes.

    To generate genetic diversity to develop resistant/tolerant varieties to Striga infestation.

    To improve capacity in efficient mutation breeding for resistance to parasitic weeds in cereals.

    Impact

    The CRP improved human capacity in mutation breeding and efficiency enhancing technologies in Striga affected members states, and the developed resistant mutant lines in the major cereals (sorghum, rice and maize) are expected to generate impact on sustainable production and food security particularly in the Striga affect countries in Africa. FAO estimates that annual crop loss due to Striga across Africa exceeds US $7 billion, impacting over 300 million people and up to 50 million hectares of crop land are infested with Striga. The developed Striga resistant varieties in the CRP will contribute significantly to reduce the loss due to the Striga across Africa and else where.

    Relevance

    The CRP is of typical relevance to the nuclear application for food and agriculture. The results generated are of relevance to integrated management of Striga parasitic weeds in cereals in affected countries mostly in Africa and part of Asia. Further more the outputs of the CRP are important in ensuring food security specially in the era of climate change as drought and consequently the effect of Striga is expected to expand and intensify. In addition, the advanced information and approach accumulated from CRP could be of relevance to scientific research related to plant-pathogen interactions.

    CRP Publications

    Japan
    Peer reviewed
    2019
    Genome sequence of Striga asiatica provides insight into the evolution of plant parasitism
    Japan
    Peer reviewed
    2018
    Induced cell fate transitions at multiple cell layers configure haustorium development in parasitic plants
    Burkina Faso/ PBGL ,Seibersdorf
    Peer reviewed paper
    2020
    Induced Resistance to Striga hermonthica in Sorghum by Gamma Irradiation. American Journal of Plant Sciences, 11, 1545-1561.
    FAO/IAEA Joint Center for NA
    Web-based success story
    2019
    Nuclear Techniques Help Develop New Sorghum Lines Resistant to the Parasitic Weed Striga
    Madagascar and PBGL
    Conference paper
    2019
    INDUCED MUTATION FOR DEVELOPING MUTANT RICE LINES TOLERANT TO THE PARASITIC WEED STRIGA ASIATICA
    Japan
    peer reviewed
    2020
    Ethylene signaling mediates host invasion by parasitic plants
    Iran/PBGL
    Conference paper
    2018
    Haploidy in Rice (Oryza Sativa L.) Mutation Breeding for Striga Resistance
    Japan
    Peer reviewed
    2020
    Subtilase activity in the intrusive cells mediates haustorium maturation in parasitic plants
    Iran/PBGL
    Conference paper
    2018
    Enhancing Efficiency of Mutation Breeding for Striga Resistance in Sorghum by Haploid Technology
    PBGL Seibersdorf
    FAO/IAEA Agriculture and Biotechnology Laboratories - NAFA Activities report
    2019
    Validated glasshouse and laboratory protocols for confirmation of Striga-resistant cereal mutants and identification of underlying resistance mechanism
    Japan/Kenya
    Peer reviewed
    2019
    The Structural Integrity of Lignin Is Crucial for Resistance against Striga hermonthica Parasitism in Rice.
    Japan
    peer reviewed
    2020
    Auxin transport network underlies xylem bridge formation between the hemi-parasitic plant Phtheirospermum japonicum and host Arabidopsis
    Japan
    Peer reviewed
    2020
    Three-dimensional reconstructions of the internal structures of haustoria in parasitic Orobanchaceae.
    Burkina Faso/PBGL
    conference paper
    2018
    Mutation Breeding for Resistance to Striga Hermonthica (Del.) Benth. in Sorghum for Food Security in Burkina Faso (ID 204)
    Japan
    Peer reviewed
    2019
    Generation is indispensable for haustorium formation of the root parasitic plant Striga hermonthica
    Japan
    peer reviewed
    2018
    Host lignin composition affects haustorium induction in the parasitic plants Phtheirospermum japonicum and Striga hermonthica
    Japan
    Peer reviewed
    2021
    Molecular dissection of haustorium development in Orobanchaceae parasitic plants.
    Iran/PBGL
    Conference paper
    2019
    Improvement of embryogenesis in anther versus shed microspore culture of rice (Oryza sativa L.)
    Japan
    Peer reviewed
    2019
    Haustorium Inducing Factors for Parasitic Orobanchaceae
    Burkina Faso and PBGL
    Conference paper
    2019
    Induced mutation in Sorghum for Striga-resistance

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