• <menu id="888u4"><menu id="888u4"></menu></menu>
  • Improving Nutritional Quality by Altering Concentrations of Enhancing Factors Using Induced Mutation and Biotechnology in Crops

    Closed for proposals

    Project Type

    Coordinated Research Project

    Project Code

    D23028

    CRP

    1487

    Approved Date

    31 March 2008

    Status

    Closed

    Start Date

    12 January 2009

    Expected End Date

    30 April 2014

    Completed Date

    28 November 2014

    Description

    In addition to providing the major calories in human diets required to sustain life, plants are also the major source of health-beneficial agents, such as vitamins and minerals required for vital physiological processes. The major staple crops however, do not provide the essential vitamins and minerals in adequate quantities and quality. A sustainable way to ensure that adequate amounts of vitamins and minerals can be obtained from diets is to develop crops that provide the required amounts in their edible tissues. Induced mutations are a proven tool in creating desirable genetic variability in plants that translate to enhanced accumulation of essential minerals; synthesis of precursors of vitamins; modified quantities and qualities of starch, proteins and oils as well as secondary plant metabolites that play critical roles in improving human health and nutrition. This CRP will utilise efficient phenotypic screens and genotypic markers to trawl through mutant collections of selected model crops to identify putative mutants in traits of interest for incorporation into breeding programmes. Both the resulting genetic resources and the methodologies for identifying them constitute the main expected outputs from this proposed CRP. It is expected that plant scientists will adopt the putative mutants and hence facilitate the processes for breeding for improved nutritional quality in staple crops. Additionally, the screening methodologies (phenotypic and molecular) that will be validated with model crops will be adapted to suit other crops and quality constraints.

    Objectives

    To improve varieties of local crops with increased yield and crop quality, enhanced levels of micronutrient contents, other nutritional factors and market-preferred traits through induced mutation techniques and supportive biotechnologies.

    Specific objectives

    To understand and identify genes involved in the biosynthesis of nutritional quality enhancing factors, and to develop efficient screening methods to facilitate the genetic improvement of nutritional quality. To transfer knowledge and technologies of beneficial mutants associated with nutritional factors from model crops to improve nutritional quality and yield into crops of socio economic importance to participating member states, specifically pepper, soybean groundnut, sweet potato, sorghum, wheat and maize.

    Impact

    ? Development of hands-on protocols for screening and analysis of mutant populations
    ? Development of new germplasm collections through mutation assisted breeding in barley, sorghum, sweet potato, maize, wheat, tomato, potato, pepper, rice, soybean
    ? Release of one rice mutant variety and two maize mutant inbred lines with improved nutritional quality
    ? Development and commercialization of products from rice, wheat and barley mutant lines with health benefits
    ? Facilitated access to advanced analytical techniques
    ? Improvement of mutation rate in tomato through application of re-mutagenesis
    ? Increased knowledge of the metabolic pathways and genes affecting phytate, carotenoids, tocopherol, flavonoids and resistant starch biosynthesis
    ? Increased knowledge of methods to improve nutritional quality of model and targeted crops
    ? Dissemination of research results to the academic community through lectures to students, presentations at workshops and conferences and 40 scientific publications (papers and book chapters)
    ? Development of national and international collaboration
    ? Dissemination of information from this CRP through outreach activities including farmers days, academic and public lectures, community workshops, public exhibitions, policy and media briefings
    ? Sharing of knowledge and expertise within the CRP for mutual benefit
    ? Production of 8 PhD theses, 19 MSc theses, 8 BSc theses, on research directly contributing to this CRP
    ? Two CSIs trained in advanced techniques in agreement holders laboratories
    ? Access to other funding sources through successes of this CRP
    ? Enabled inter-disciplinary collaborations between crop scientists and nutritionists/health professionals

    Relevance

    Improving the delivery and bioavailability of essential vitamins and minerals to address nutritional deficiencies in an increasing number of people worldwide

    CRP Publications

    Type

    Scientific publication in peer reviewed journal

    Year

    2012

    Description

    Seibt KM, Wenke T, Wollrab C, Junghans H, Muders K, Dehmer KJ, Diekmann K, Schmidt T (2012) Development and application of SINE-based markers for genotyping of potato varieties. Theor Appl Genet 125(1):185-96

    Country/Organization

    Germany

    Type

    Book

    Year

    2014

    Description

    Mutagenesis: Exploring Novel Genes and Pathways. (2014) Tomlekova N.B., Kozgar M.I., Wani M.R. (Eds), hardback, edited volume ISBN13: 978-90-8686-234-4; ISBN10: 9086862349, Publication Date: 2/6/2014, Publisher(s): Wageningen Academic Publishers, The Netherlands, Enfield Pub & Distribution Co, ± 500 pages.

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2013

    Publication URL

    http://dx.doi.org/10.1016/j.mrfmmm.2013.05.002

    Description

    Luke Mehlo,*, Zodwa Mbambo, Souleymane Bado, Johnson Lin, Sydwell M. Moagia,Sindisiwe Buthelezia, Stoyan Stoycheva, Rachel Chikwamba (2013) Induced protein polymorphisms and nutritional quality, Mutation Research, Elsevier B.V.,

    Country/Organization

    South Africa (CSIR)

    Type

    Book

    Year

    2013

    Description

    Poryazov, I.B., Petkova, V.P., Tomlekova, N.B. (2013) Vegetable production, ‘DIMI 99’ Ltd., Sofia, ISBN: 978-954-765-064-0, 318 p.

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2010

    Description

    Nikolova, V., Todorova V., Stefanova Y., Tomlekova N. (2010) Cytological particularities in nuclear and nuclear-cytoplasmic male sterile pepper lines, Caryologia, 63:3, 262-268.

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2009

    Description

    Shu X, Limeng Jia, Hongxia Ye, Chengdao Li, Dianxing Wu. Slow digestion properties of rice differential in resistant starch. Journal of Agricultural and Food Chemitry. 2009, 57: 7552-7559

    Country/Organization

    China

    Type

    Book Chapter

    Year

    2012

    Publication URL

    http://www.intechopen.com/articles/show/title/genetic-diversity-of-bulgarian-pha…

    Description

    Tomlekova, N. (2012) Genetic diversity of Bulgarian Phaseolus vulgaris L. germplasm collection through phaseolin and isozyme markers. In: ?aliskan M. (Ed), The Molecular Basis of Plant Genetic Diversity, http://www.intechopen.com/books/the-molecular-basis-of-plant-genetic-diversity, pp. 374, ISBN 978-953-51-0157-4, Rijeka: InTech. pp. 181-230

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2010

    Description

    Tomlekova, N.B. (2010) Induced mutagenesis for crop improvement in Bulgaria. Plant Mutation Report, 2(2): 1-32.

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2011

    Description

    da Silva, L.S., Jung, R., Zhao, Z.-y., Glassman, K., Grootboom, A.W., Mehlo, L., O'Kennedy, M.M., Taylor, J., Taylor, J.R.N. 2011. Effect of suppressing the synthesis of different kfirin subslasses on grain endosperm texture, protein body structure and protein nutritional quality in improved sorghum lines. Journal of Cereal Science 54 (1): 160-167.

    Country/Organization

    South Africa (CSIR)

    Type

    Scientific publication in peer reviewed journal

    Year

    2013

    Description

    Mark AB, Kápolna E, Laursen KH, Halekoh U, Rasmussen SK, Husted S, Larsen EH, Bügel S. Consumption of organic diets does not affect intake and absorption of zinc and copper in men - evidence from two cross-over trials. (2013) Food Funct. 4(3):409-19.

    Country/Organization

    Denmark

    Type

    Scientific publication in peer reviewed journal

    Year

    2012

    Description

    Balacheva, E., Kartzeva T., Atanassova B., Tomlekova N. (2012) The Diversity in Tomato Colours – Potential for Diversity in Tomato Nutritive Quality. Acta Horiculturae, 960, 31-36.

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2011

    Description

    Wenke T, D?bel T, S?rensen TR, Junghans H, Weisshaar B, Schmidt T (2011) Targeted identification of short interspersed nuclear element families shows their widespread existence and extreme heterogeneity in plant genomes. Plant Cell 23(9):3117-28

    Country/Organization

    Germany

    Type

    Scientific publication in peer reviewed journal

    Year

    2010

    Description

    Rasmussen SK, Ingvardsen CR, Torp AM. Mutations in genes controlling the biosynthesis and accumulation of inositol phosphates in seeds. (2010) Biochem Soc Trans. 38(2):689-94. (Review).

    Country/Organization

    Denmark

    Type

    Scientific publication in peer reviewed journal

    Year

    2011

    Description

    WHITE, P.J., BROADLEY, M.R., Physiological limits to zinc biofortification of edible crops, Frontiers Plant Sci. (2011) 2:80.

    Country/Organization

    UK

    Type

    Book Chapter

    Year

    2014

    Description

    Tomlekova, N. (2014) Use of mutant genes for exploring and altering carotenoid biosynthetic pathway of sweet pepper: The Bulgarian viewpoint. In: Tomlekova N.B., Kozgar M.I., Wani M.R. (Eds), Mutagenesis: Exploring Novel Genes and Pathways, Wageningen Academic Publishers, The Netherlands, 433-454.

    Country/Organization

    Bulgaria

    Type

    Conference Proceedings

    Year

    2012

    Description

    N Chiloane, N Myeza, S Laurie, M Malebana. Application of in vitro techniques in mutation breeding of sweetpotato. 30th SA Society of Agric Technologists Congress, Salt Rock Hotel, Durban, 18-21 Sep 2012.

    Country/Organization

    South Africa

    Type

    Scientific publication in peer reviewed journal

    Year

    2013

    Description

    Roos N, S?rensen JC, S?rensen H, Rasmussen SK, Briend A, Yang Z, Huffman SL. Screening for anti-nutritional compounds in complementary foods and food aid products for infants and young children. Matern Child Nutr. 2013 Jan;9 Suppl 1:47-71.

    Country/Organization

    Denmark

    Type

    Conference Proceedings

    Year

    2014

    Description

    Spasova-Apostolova, V.V., Tomlekova, N.B., Todorova, V.Y., 2014. Conducting marker-assisted selection in pepper for increasing quality and selection of donors for F1 hybrids. 30-31.10.2013, Scientific works. Union of Scientists in Bulgaria - Plovdiv. Series “Techniques and technologies”. Proceeding of the conference of Union of the Scientists of Bulgaria – Plovdiv, pp. 21-26.

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2010

    Description

    Draganov, M., Miloshev G., Popov N., Murdjeva M., Staneva D., Kostova M., Draganov D., Tomlekova N. (2010) Molecular and cytogenetics criteria for identification of serum-free cell cultures. Biotechnology & Biotechnological Equipment, 24: 139-143.

    Country/Organization

    Bulgaria

    Type

    Conference Proceedings

    Year

    2009

    Description

    LAURIE, S.M., et al., Initiation and first results of a Biofortification program for sweet potato in South Africa. J. Crop Improvement 23 (2009) 235.

    Country/Organization

    South Africa

    Type

    Scientific publication in peer reviewed journal

    Year

    2012

    Description

    WHITE, P.J., et al., Biofortification of potato tubers using foliar zinc-fertilisers, J. Hort. Sci. Biotechnol. 87 (2012) 123-129.

    Country/Organization

    UK

    Type

    Conference Proceedings

    Year

    2009

    Description

    LAURIE, S.M., et al., Quick screening method for drought tolerance for sweet potato tested in South Africa. Triennial Symposium of International Society of Tropical and Subtropical Crops (ISTRC), 2-6 Nov 2009, CIP-HO/UANALM, Lima, Peru (2009).

    Country/Organization

    South Africa

    Type

    Conference Proceedings

    Year

    2012

    Description

    Migdalis, N., Serdaris P., Tomlekova N. (2012) Desert Challenge for Western Brands: the United Arab Emirates Experience. 1. The emirates as a place of internal commerce and behavior of one of the components of the market – the users. Proceedings of the International Conference on Management and Entrepreneurship, Technical University, 19 - 20 October 2012, Plovdiv, 164-169.

    Country/Organization

    Bulgaria

    Type

    Book Chapter

    Year

    2012

    Publication URL

    http://www.globalsciencebooks.info/JournalsSup/12BBB_6_SI1.html

    Description

    Tomlekova, N., Yancheva, S., Balacheva, E. and Atanasova, B. (2012) Molecular identification of tomato mutant lines. In: Kozgar, M.I. and Khan, S. (eds.) Induced mutations in crops. Bioremediation, Biodiversity and Bioavailability 6 ((Special Issue)), Global Science Books, Ltd., UK, (Special Issue 1): 58-64 (Print ISSN 1749-0596), book link:

    Country/Organization

    Bulgaria

    Type

    Book

    Year

    2014

    Description

    Mutagenesis: Exploring Genetic Diversity in Crops. (2014) Tomlekova N.B., Kozgar M.I., Wani M.R. (Eds), Edited by Nasya Tomlekova, Imran Kozgar and Rafiq Wani (Editors), hardback, edited volume ISBN13:; ISBN10: 9086862349, Publication Date: 15/6/2014, Publisher(s): Wageningen Academic Punblishers, The Netherlands, Enfield Pub & Distribution Co, ± 400 pages

    Country/Organization

    Bulgaria

    Type

    Conference Proceedings

    Year

    2012

    Description

    Migdalis, N., Serdaris P., Tomlekova N. (2012) Desert Challenge for Western Brands: the United Arab Emirates Experience. 2. Possibilities of the European brands to sell on the emirates’ markets. Proceedings of the International Conference on Management and Entrepreneurship, Technical University, 19 - 20 October 2012, Plovdiv, 170-175.

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2009

    Description

    WHITE, P.J., BROADLEY, M.R., Biofortification of crops with seven mineral elements often lacking in human diets - iron, zinc, copper, calcium, magnesium, selenium and iodine, New Phytol. 182 (2009) 49-84.

    Country/Organization

    UK

    Type

    Conference Proceedings

    Year

    2012

    Description

    M.E. Malebana, S.M. Laurie, N. Chiloane, N. Myeza & R. Kleynhans. Radio-sensitivity results towards induced mutations for improved nutritional quality and drought tolerance in sweet potato (Ipomoea batatas L. LAM). SA Plant Breeders Symposium, 11-14 Mar 2012, Skukuza.

    Country/Organization

    South Africa

    Type

    Scientific publication in peer reviewed journal

    Year

    2013

    Description

    Petrov, V., Denev I., Draganov M., Timin O., Panchev I., Tomlekova N. (2013) Molecular characterization of advanced mutants for early detection of high beta-carotene concentrations in pepper breeding programmes. Comptes rendus de l'Académie bulgare des sciences, 66(2), 303- 310

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2012

    Description

    Nacheva, E., Tomlekova, N., Petkova, V., 2012. Induced mutagenesis in F1 hybrid seeds of potato. Journal of Ecology and Future, vol. XI, ? 4, 30-35.

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2011

    Description

    Timina, O.O., Timin O.Y., Fiodoroff S.K., Tomlekova N. (2011) Inheritance of pericarp color pattern and ?-carotene content in vegetable pepper. Vavilov Journal of Genetics and Breeding, /In Russian/, 15(3): 585-594.

    Country/Organization

    Bulgaria

    Type

    Conference Proceedings

    Year

    2009

    Description

    Gómez-Pando, L.; Eguiluz, A.; Jimenez, J.; Falconí, J; Heros E. 2009. Barley (Hordeunvulgare) and Kiwicha (Amaranthuscaudatus) Improvement by Mutation Induction in Peru. Q.Y. Shu (ed.), Induced Plant Mutations in the Genomics Era. Food and Agriculture Organization of the United Nations, Rome. 371-374.

    Country/Organization

    Peru

    Type

    Scientific publication in peer reviewed journal

    Year

    2013

    Description

    Shu X, Jianwei Xu, Ying Wang, S?ren K. Rasmussen, Dianxing Wu. Effects of gamma irradiation on starch digestibility of rice with different resistant starch content. (2013) International Journal of Food Science & Technology, 48, 35-43

    Country/Organization

    China/Denmark

    Type

    Scientific publication in peer reviewed journal

    Year

    2014

    Description

    Petkova, V., Todorova V., Tomlekova N. (2014) Efficiency of the photosynthetic apparatus of sweet pepper (Capsicum annuum L.) F1 hybrids under high temperature stress, Plant Sciences (Bulgaria) 51: 43-48

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2014

    Description

    Shu X, Rasmussen SK. Quantification of amylose, amylopectin, and ?-glucan in search for genes controlling the three major quality traits in barley by genome-wide association studies. (2014) Front Plant Sci. 15;5:197. doi: 10.3389/fpls.2014.00197.

    Country/Organization

    China/Denmark

    Type

    Scientific publication in peer reviewed journal

    Year

    2009

    Description

    WHITE, P.J., et al., Relationships between yield and mineral concentrations in potato tubers, HortScience 44 (2009) 6-11

    Country/Organization

    UK

    Type

    Book Chapter

    Year

    2014

    Description

    Tomlekova, N., Timin, O., Arnaoudova, Y., Timina, O., Wani, M.R., Kozgar, M.I. (2014) Trends and achievements in F1 hybrids of sweet pepper utilizing induced male-sterility In: Tomlekova N.B., Kozgar M.I., Wani M.R. (Eds), Mutagenesis: Exploring Genetic Diversity in Crops, Wageningen Academic Publishers, The Netherlands, 13-38.

    Country/Organization

    Bulgaria

    Type

    Scientific publication in peer reviewed journal

    Year

    2012

    Description

    Shu X, Gunter Backes, S?ren K.Rasmussen. Genome-wide association study of resistant starch (RS) phenotypes in a barley variety collection. Journal of Agricultural and Food Chemistry. 2012, 60: 10302-10322

    Country/Organization

    China/Denmark

    Type

    Scientific publication in peer reviewed journal

    Year

    2012

    Description

    WHITE, P.J., BROADLEY, M.R., GREGORY, P.J., Managing the nutrition of plants and people, Appl. Environ. Soil Sci. 2012 (2012) article 104826

    Country/Organization

    UK

    Type

    Book Chapter

    Year

    2014

    Description

    Tomlekova, N., Panayotov, N., Kozgar, M.I., Wani, M.R., Serdaris, P., Balacheva, E. (2014) Inducing and exploring new mutant donors of tomato. In: Tomlekova N.B., Kozgar M.I., Wani M.R. (Eds), Mutagenesis: Exploring Genetic Diversity in Crops, Wageningen Academic Publishers, The Netherlands, 285-308.

    Country/Organization

    Bulgaria

    Stay in touch

    Newsletter

    午夜爱爱爱爱爽爽爽视频网站